KMID : 0368420070500040514
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Journal of Plant Biology 2007 Volume.50 No. 4 p.514 ~ p.514
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Mechanism by whichBacillus-Derived 2-Aminobenzoic acid inhibits the growth ofArabidopsis thaliana Roots
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Hoang Lam
Song Kyung-Sik Rhee In-Koo Kim Jeong-Hoe Lee Sang-Man
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Abstract
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To analyze the growth inhibitory mechanism of a 2-aminobenzoic acid (2-AA) derived fromBacillus cereus EJ-121, we treatedArabidopsis thaliana plants with 2-AA, 2-AA analogs, auxin (NAA), a known auxin transport inhibitor [2,3,5-triiodobenzoic acid (TIBA)], and an ethylene action inhibitor [silver thiosulfate (Ag)]. Root development was significantly inhibited by 50 ¥ìM 2-AA, whereas the growth of bacteria and yeast was undeterred. The application of two 2-AA analogs - 3-aminobenzoic acid (3-AA) and 4-aminobenzoic acid (4-AA) - did not impairArabidopsis root growth at concentrations below 100 ¥ìM. These results suggest that the effect of 2-AA is not due to its chemical structure, but because of its conversion to another metabolite, IAA. To confirm this, we supplemented TIBA in the growth medium, and found that the degree of inhibition was significantly reduced. Similarly, when plants were co-treated with 100 ¥ìM Ag, the negative effect of 50 ¥ìM 2-AA was greatly diminished. All of these observations support the proposal that this inhibition results from the conversion of 2-AA to IAA. Furthermore, the increased auxin level leads to a rise in ethylene synthesis, which then blocks root growth and, ultimately, retards overall plant development.
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KEYWORD
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2-aminobenzoic acid, auxin, ethylene, plant growth inhibitor
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